Here are a list of characteristics associated with this protein. I will be grateful if anyone can make something out of this.
-Very different concentration when measured with BCA and Biorad assay (both assay use BSA as standard).
-Heat supernatant does not work with Biorad assay unless DNAase and RNAase are added (possible protein-DNA complex formation after the heat step)
-Heat supernatant is fairly clean, but the residual proteins are impossible to get rid of using column or ammonium sulfate precipitation.
-The salted out protein has a gelatin like consistency.
-This is a basic protein, and is not stable in pH<7. The buffer I use now is Tris-HCl pH 8.0.
-After breaking the cell, there are some soluable protein, and some inclusion bodies (50:50).
-The protein precipitate when it is >1.5 mg/ml. (tried adding metal, salt, and glycerol).
http://biowww.net/mynews/tree.php?group_name=bionet.molbio.proteins&begin=0
