thanks for the hint.
i looked it up - but was not able to find out what ccdB is.... what kind
of protein is coded by this insert? how works negative selection with it?
Tom Knight schrieb:
> Invitrogen uses the ccdB gene which is toxic in E. coli as a way of
> doing positive selection of insertions in its cloning systems. If you
> look at their vector sequences you can find the insertion, and you
> could easily get a copy of the gene from any one of a number of their
> vectors. I think they even sell a resistant strain of coli, so that
> you can grow it.
