On 14 Dec 2003 13:45:21 -0800, dikla_dar at walla.co.il (diki) wrote:
>my question is: in the plasmid pBR322,
Wow! Blast from the past.!
>if I replace the whole gene for
>resistance to amp. in a certain insert, is there a good chance that
>the new gene will be expressed as a protein?
Depends. You got an E coli promoter on that insert?
> what are the
>circumstances in which it will happen? thank u very much for
There are a lot better vectors these days to express proteins in coli.
Or is this an exam question?
nick_theodorakis at hotmail.com
nicholas_theodorakis [at] urmc [dot] rochester [dot] edu