how can I improve the purification of protein

Kyle Legate legatek at hotmail.com
Fri Aug 29 09:28:22 EST 2003

haidongtan at 163.com wrote:
>  How can I get single peak using HPLC analyzing from my purified
> protein? The purity of  target protein cant reach up to 98% according
> to biological material standard  for there always is a small peak
> which maybe occupy about 4% on the whole area.I exceedingly hope some
> experts on the protein downstream can give me some suggestion and
> thoughts and ideals for that.
If the contaminating peak is of a different molecular weight than your
protein of interest, you could include a gel filtration step in your

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