haidongtan at 163.com wrote:
> How can I get single peak using HPLC analyzing from my purified
> protein? The purity of target protein cant reach up to 98% according
> to biological material standard for there always is a small peak
> which maybe occupy about 4% on the whole area.I exceedingly hope some
> experts on the protein downstream can give me some suggestion and
> thoughts and ideals for that.
>If the contaminating peak is of a different molecular weight than your
protein of interest, you could include a gel filtration step in your
purification.
