tissue homogenization

Scott Coutts scott.coutts at med.monash.edu.au
Wed Aug 20 06:39:18 EST 2003

Oh, sorry, I forgot to mention something... I cant remember what the 
little eppy type mortar and pestles were called or who sold them!

What about sonication?

Scott Coutts wrote:
> Hmm... dont know about the BEST way, but you can buy little 'mortar and 
> pestle' type things that fit exactly into the bottom of an eppendorf 
> tube. We used them a while back for crushing and homogenising individual 
> Drosophila flies.
> As for the solvent, well, I guess that depends on how you have your 
> ELISAs set up.
> julie.decock at med.kuleuven.ac.be wrote:
>  >
>> what is the best method for homogenization of very small 
>  > amounts (2-18 mg) of mammary tumor tissue for ELISA and
>  > in which solvent should this be done?

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