IUBio

how to elute a protein from PVDF membrane

shuanyi shuanyi at hotmail.com
Wed Apr 30 07:18:40 EST 2003


Could anyone provide me a method to elute a protein from PVDF
membrane?
Will it work if i use the acetonitrile? 
I've heard someone would use acid (like formic acid or TFA) at the
same time. What is the main purpose that use the acid?

Any idea warmly received.

Best regards,

ShuanYi



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