Problem with concentrating His Tag protein

[D.K.]

In article <b8mjij$i2d$1 at agate.berkeley.edu>, lhom at OCF.Berkeley.EDU (Louis Hom) wrote:
>
>I'm not sure what volumes you're working with, but it may make sense to 
>dialyze against some low ionic strength buffer for a short time before 
>concentrating to avoid the problems DK mentioned.

With some proteins though, exactly opposite (higher salt 
concentration) is needed to keep them in solution. 

DK