My recombinant protein is strongly expressed in inclusion bodies in E coli. I have heard that purified inclusion bodies can be directly injected into rabbits for antibody production without the need for solubilising and refolding. Has anyone tried this and / or have any hints and tips for me?
Many thanks,
Tania Humphrey
http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
