i have just started doing westerns recently. i am facing problems with the PVDF membrane that
we use. i soak it in methanol & before it dries i soak it in ddwater & then in the western transfer
buffer.
The transfer looks good--as i am able to see all the lanes & even the marker . The problem is
that after i block the membrane in 5% milk ( carnation non-fat dry milk powder & made in 1X
TBS) for an hour or even overnite in a cold room---the membrane refuses to wet---it looks as if
its dry, only the egdes seem to be wet.
Anyone with a solution---will be greatly appreciated.
thank you
aditi
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