I am trying to quantify the total HCP in a protein bulk sample.
I am doing an HCP ELISA as well as Slot Blot.
My values(HCP Concentration) by ELISA are coming very high(about 100times more) as compared to Slot Blot.
My sample is a conc protein..about.7mg/ml
Has anyone ever experienced this kind of discrepancy?
Any inputs on what could be going on. Could the protein interfere in SlotBlot and not in ELISA.
I guess an ELISA is more sensitive,I know. But do we expect 100 fold difference?
The slot Blot shoul be at least somewhere near the the ELISA values I guess.
I hope someone can throw light on this issue.