IUBio

CAPS or Tris-Gly?

James Bassuk bassuk at u.washington.edu
Thu Sep 19 23:38:46 EST 2002


On Sun, 25 Aug 2002, News wrote:

> Are there any differences in the efficiency between CAPS and Tris-Gly/MetOH
> buffers for Western blotting?
>
>

CAPS is often used to blot to PVDF -- the lack of glycine here eliminates
downsteam sequencing contamination




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