Barak Akabayov wrote:
> Dear all:
> I have a protein 50 kD with certain pi.
> Normally it is prepared within Hepes buffer pH 6.8 and ionic strength of
> 200mM (NaCl), unfortunately when I concentrate the protein after
> purification (above 10uM) it precipitate. How can I avoid it?
> Much appreciate any help
> Barak
>barak_akabayov at yahoo.com
Try different buffers - once I was not able to dissolve a protein after lyophylisation in 0.05M phosphate buffer pH7, which I was using during whole purification procedure, but protein was dissolved in 0.05M ammonium bicarbonate without any problem up to concentration 20mg/ml
http://biowww.net/mynews/tree.php?group_name=bionet.molbio.proteins&begin=0
