Artem Evdokimov wrote:
> I am assuming that you have already checked for all sorts of 'simple'
> problems, such as spontaneous mutations in the promotor region, frameshifts,
> etc. Could it be a nasty case of proteolysis ?
I have sequenced gene - RBS included- to be sure, that there is not frame shift or any other mutations. I have not sequenced promoter region – I can do that.
Lately I have heard about adding protease inhibitor PMSF directly into medium. Does it make any sense?
http://biowww.net/mynews/tree.php?group_name=bionet.molbio.proteins&begin=0
