Has anyone experience of purification of yeast alcohol dehydrogenase using
the dye ligand Cibacron Blue F3GA? I have tried it many times without
success. Start/binding buffer 20mM Tris, 5mM mercaptoethanol, 5mM MgCl2,
0.4mM EDTA, as described in several protocols but ADH doesn't bind! Plenty
of ADH activity. Any help appreciated as lab class approaches!
--
Fergus Doherty,
School of Biomedical Sciences,
Nottingham University,
Fergus.Doherty at nottingham.ac.uk
0115 970 9366 (74-41366 internal)
