Dear Katrin
How much % of gel you are using. Plz check your Acrylamide and find out the % of it to be used. This may solve your problem. There is no problem for you if you load membrane proteins or whole cell extracts or even inclusion bodies.Maintain costant voltage while running a gel. Use fresh running buffer.
Regards,
kiran kumar velpula
Katrin Westphal wrote:
> Hi,
> I have problems with my SDS-PAGE: The bands grow wider the further they
> have run and the resolution grows bad: bands at the the top seem to be
> ok, while those at the bottom are blurred and much bigger than the ones
> above.
> My samples are bacterial membrane proteins or whole cell extracts in
> sodiumphosphate buffer.
> Thanks in advance for any help!
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