best fusion tag for protein purification?

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Fri Sep 6 11:03:14 EST 2002

> I am looking for the best (on average) affinity tag for efficient
purification of recombinant proteins expressed in E. coli. Am using one > of
the pET series of expression vectors if that helps.

MBP-cleavage site-protein of interest-Hisx6 sandwich is very good. We use
TEV protease cleavage site for obvious reasons. This sandwich is trivial to
construct. As to expression strain - usually BL21 DE3 with RIL or Rosetta
rare codon booster works well.


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