Carol:
try prep. isoelectric focusing pH range 4 to 6 for ex.
Sincerely
Pier Carlo
^^^^^
Pier Carlo Montecucchi
MONTEGEN
<chengzhjun at hotmail.com> wrote in message
news:20021022055838.24989.qmail at ww02.hostica.com...
> my protein is about 30kDa and its pI is 5.1. I used pH=8.0 PBS binding
buffer to banlance the Ni-His tag column. but when i put the protein
solution into the column,the protein solution becomes turbid and the resin
aggregates together and so the protein buffer could't flow down. How can I
do with it .
> Any information would be appreciated!
> Carol
>>>http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
