Hi, maybe a silly question but my experience in Proteins is poor.
I need to perform a IEF of my bacterial extract (semipurified) but I
do not which is the best buffer to use with my sample, I have
precipitated my sample (acetone) and resuspended with H2Od but the
sample doesn´t run in the gel, it stay in the same place (the standars
run well) no matter where I put the sample (acid or basic side)(using
Phast system gel).
Thanks in advence.
