FYI
I stained a 2D gel with sypro ruby and afterwards with silver staining and
I got the same effect ...
Strange eh
<beismann at em.uni-frankfurt.de> wrote in message
news:20021010142432.2660.qmail at ww02.hostica.com...
> Hello,
>>>> I have done silver stains of SDS gels many times before but last time
something strange occured: I got an inverse stain with dark background and
light protein bands (altogether rather faint). Before this stain, I prepared
fresh thiosulfate buffer, and formaldehyde was freshly added to the silver
solution and the developing solution.
>> What can be the cause of the inverse staining, an thus, how can I get back
positive stains?
>>>> Thanks for helping me,
>>>> Silke
>>>>>>http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
