rpg at mrc-lmb.cam.ac.uk wrote:
>In article <ab7fes$6nl$2 at news.doit.wisc.edu>,
>dk at no.email.thankstospam.net (D.K.) wrote:
>>> Scott <scj-13 at deletemecharter.net> wrote:
>> >I need to express a 5 KDa and a 10 KDa protein in E. coli. I've tried a
>> >number of candidates, but they always express very poorly.
>> >Interestingly, if I clone two tandem copies(of a 10 KDa) of the same
>> >thing it works very well. E. coli seems to not really tollerate such
>> >small protein molecules very well.
>>>> This is a well-known fact.
>>Really? My 7.5 kDa protein expresses just fine - in fact, incredibly
>well - in E. coli. So well that I managed to solve its structure by
>homonuclear NMR (see last month's NSB).
>
You know that there are no hard rules, right? :-)
Generally though, everything below 5K expresses poorly on its own.
DK
