In article <ab7fes$6nl$2 at news.doit.wisc.edu>,
dk at no.email.thankstospam.net (D.K.) wrote:
> Scott <scj-13 at deletemecharter.net> wrote:
> >I need to express a 5 KDa and a 10 KDa protein in E. coli. I've tried a
> >number of candidates, but they always express very poorly.
> >Interestingly, if I clone two tandem copies(of a 10 KDa) of the same
> >thing it works very well. E. coli seems to not really tollerate such
> >small protein molecules very well.
>> This is a well-known fact.
Really? My 7.5 kDa protein expresses just fine - in fact, incredibly
well - in E. coli. So well that I managed to solve its structure by
homonuclear NMR (see last month's NSB).
r
--
Richard P. Grant 0x5F9559B1 MRC Lab of Mol Biol
rpg 'at' mrc-lmb.cam.ac.uk New: http://www.rg-d.com/BioLOG/
When MS created NT, how did they get an infinite number of monkeys?
