John Philo wrote:
> Susanne and all,
>> I'll jump in late and offer a possible different interpretation.
>> Inferring masses from size-exclusion chromatography is always subject to
> large errors---I have seen so many cases where a putative dimer is really a
> monomer, and also a case where a protein said to be a monomer by SEC was
> really a trimer. If you don't mind being off by factors of 2-3 either way,
> SEC is fine.
Oh dear, I didn't know that.
does it help that I get two peaks with pure protein, one corresponding quite
exactly to a monomer and the other to a dimer? I did run commercial standards.
I agree I have no idea about their structure.
I will get back to your company when i need accurate analysis, thanx :)
Susanne
