Hi everyone,
I have a couple of questions about removal of triton series detergents
from solutions.
>From the research I've done so far it seems that the most common method
is the use of BioRad's BioBeads SM2. I've read a few newsgroup posts and
articles talking about extensive washing in acetic acid, acetone, water
etc. Some people suggest this acetic acid/acetone/water washing as "per
the manufacturers instructions"... our biobeads say that they're ready
to use... does anybody know what the purpose of the acetic acid and
acetone is, and whether biorad has changed the formulation so that this
washing is no longer needed?
Also, what about Amberlite XAD? Has anybody used this for removal of
TX-100 or TX-114? They're also polystyrene beads, so I assume they will
achieve the same thing. But they're about 10 times cheaper than the
biobeads. Are they less effective? Do they have less surface area?
What's the reason that they're so much cheaper? Is there a protocol
around or can they just be added and incubated as per biobeads? The
product sheet doesnt really describe their use, other than that they
should be 'conditioned' prior to use with water.
Lastly... any other resins/beads that can be used effectively?
Thanks.
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