I used continous flow dialysis against buffer (TRIS or Phosphate
buffer PH 7) for changing buffer. The time isn't important.
"Artem Evdokimov" <AEVDOKIMOZ at cinci.rr.com> wrote in message news:<9g_B8.58555$Ez5.14761633 at typhoon.neo.rr.com>...
> Detergents ? Continuous flow dialysis ? Dialysis via ultracentrifugation ?
>> it really depends on the details - how long do you dialyze, against what
> (and from what) etc.
>> A.G.E.
> "Mariella" <parisi at fis.unipr.it> wrote in message
> news:fad5fa87.0205070655.3e5eafde at posting.google.com...> > Hi everybody!
> > I've a problem. The protein I study gets aggregated when I put it in
> > dialysis. I've tried many things: to change the buffer, collodion
> > bags,
> > to add EDTA or Glycerol or Salt. But nothing.
> > By X-ray it seems that nothing binds it, and so I've rejected the idea
> > that
> > the protein loses a ligand in dialysis.
> > The protein is the porcine Odorant Binding Protein (a Lipocalin): its
> > analogous one ( that one extract from bovine) doesn't have this
> > behaviour.
> > What can I do?
> >
> > Thanks
> >
> > Mariella Parisi
