Detergents ? Continuous flow dialysis ? Dialysis via ultracentrifugation ?
it really depends on the details - how long do you dialyze, against what
(and from what) etc.
A.G.E.
"Mariella" <parisi at fis.unipr.it> wrote in message
news:fad5fa87.0205070655.3e5eafde at posting.google.com...
> Hi everybody!
> I've a problem. The protein I study gets aggregated when I put it in
> dialysis. I've tried many things: to change the buffer, collodion
> bags,
> to add EDTA or Glycerol or Salt. But nothing.
> By X-ray it seems that nothing binds it, and so I've rejected the idea
> that
> the protein loses a ligand in dialysis.
> The protein is the porcine Odorant Binding Protein (a Lipocalin): its
> analogous one ( that one extract from bovine) doesn't have this
> behaviour.
> What can I do?
>> Thanks
>> Mariella Parisi
