Hi everybody!
I've a problem. The protein I study gets aggregated when I put it in
dialysis. I've tried many things: to change the buffer, collodion
bags,
to add EDTA or Glycerol or Salt. But nothing.
By X-ray it seems that nothing binds it, and so I've rejected the idea
that
the protein loses a ligand in dialysis.
The protein is the porcine Odorant Binding Protein (a Lipocalin): its
analogous one ( that one extract from bovine) doesn't have this
behaviour.
What can I do?
Thanks
Mariella Parisi
