IUBio

Protein solubility

Peter Cherepanov peter.cherepanov at uz.kuleuven.ac.be
Fri May 3 09:48:06 EST 2002


about truncations - if you want to make your protein soluble, it seems
reasonable to me to first identify independent domains in it (by limited
proteolisis?) . Most probably it will be better to chop your protein between
the domains, it will increase the chances to obtain a soluble deletion and
with more defined structure.

also check for cysteins, often they contribute to aggregation as well:
nocking out some Cys residues you may eliminate problems with slow
aggregation and precipitation of your protein.

Peter

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