Philipp Wechner <philipp.wechner at uibk.ac.at> schrieb:
> > If they do lose the plasmid from storage in glycerol stocks - how does
> > this occur? What does the glycerol do?
>> I kept some of my transformed BL21 in glycerol in the past - but i stopt
> doing this because it is much faster to just keept the Miniprep and
> transform when you need it....
> I never encountered problems with my stocks in the past
Indeed. Some weeks ago, I thawed a glycerol stock of BL21 from 1996
because we couldn't find the plasmid DNA (of the respective mutant). It
had had to stand two lab moves, each time beeing transferred from -70°C
to dry ice and back. They grew and even expressed just fine, but I
preferred to isolate DNA and retransform.
Bye, Frank
--
> But I don't really see running SETI at Home as practical as Folding at Home. What,
> exactly, would be the benefit of finding intelligent aliens on the other side
> of the galaxy?
Maybe they're broadcasting the principles of protein folding... [from bionet.*]
