fluorescence quenching of tryptophan

[kerstin.janisch at bbsrc.ac.uk]

> > Dear Kerstin,
>                      In order to monitor the fluorescence
> quenching, the protein sample should be purified. It is very
> likely that you have impurities in the protein sample, that
> might interefering and creating the problem.
> Jawaid
> > 
> > 
Dear Jawaid

i think my protein is pure, but what purity do you recommend?
cheers,
kerstin
http://www.biowww.net/index.php/forum/messagelist/8/