> i've got a problem with the fluorescence quenching of tryptophan in
proteins due to binding other molecules. the assay is not reproducable. does
anybody know something about this topic and can offer some help? the
important bit is, is the binding and therefore the quenching temperature
depending? what else can influence this system?
> thanks a lot! it's really urgent.
> cheers,
> kerstin
This quenching should be veyr site- and molecule-specific. Experiments of
this sort require high accuracy, since even minor perturbations in the setup
can change the effect dramatically. I second Emir's point that we'd need
much more information about what is it that you're trying to do before we
could try to help you in more detail.
A.G.E.
