I now search for a good extraction method for lyze the intact cells.
The sample is pretty complex, including fungi(yeast or/and spores),
bacteria, virus and some other microbes. I am not quite sure if all of
them will present in the sample, but hopefully, they will appear in
different fractions (bacteria and fungi ocuppy most).
First, I am looking for a method which just break the cells and
extract the soluble protein in the cells.
After that, I also need a method which not only extract the soluble
protein but also insoluble proteins(e.g. membrane proteins). I know
there are some insoluble proteins which can not be extracted. I think
the method which extracts the proteins as many as possible is a
Thanks in advance for your great help.