Dear Hans,
HI.
You may find it useful. Following articles are about inclusion bodies
in Yeast, bacteria & even animal cells too.
Saccharomyces: Cousens,L., et al (1987), Gene,61,256
Bacillus: Parante,D.,et al (1991) FEBS lett.,77, 243
Insect cell lines: Thomas CP, et al (1990). J.Gen.Virol.,71,2073
Hope you will find them useful
S.Ballal
Indus Biotherapeutics Ltd.
http://www.indusbio.co.in/
Philipp Wechner <philipp.wechner at uibk.ac.at> wrote in message news:<3D11990E.4182F636 at uibk.ac.at>...
> Hans Kiefer schrieb:
>> > Hi
> >
> > I wonder whether anybody is aware of inclusion body formation in a
> > eukaryotic expression systems such as Pichia, Saccharomyces or
> > Baculo/Sf9 cells.
> >
> > What I am interested in is high-level protein expression in an
> > aggregated, non-functional state, since we deal with protein
> > refolding.
>> If you whish IB - why not express in E. coli? if you want to do a refold
> afterwaards this should be no problem (unless your protein has to be
> modified posttranslationally)
> I am working on the refolding of an eucaryontic proteine from IB in coli
> - and it works well.
>> I guess that it is also possible to induce IB in eucaryontic cells - i
> think i heared of IB in yeast. Depends on the strenght of the promotor
> and the protein you express.
>>> i know that this answer is not too much help, sorry :)
>> >
> >
> > Does it occur at all, can it be influenced by strong/weak promotors,
> > growth temperature etc.?
> >
> > Thanks for your help!
> >
> > Hans
