This peptide is a perfect candidate for ion exchange purification, something a
lot of peptide chemists seem to forget about nowadays.
In article <20020702215153.21164.qmail at ww02.hostica.com>, shibbu_r at yahoo.com
wrote:
>>I HAVE A PROBLEM WITH THE PURIFICATION OF 26 AMINOACID PEPTIDE PREPARED BY
> SOLID PHASE PEPTIDE SYNTHESIS. THE SEQUENCE IS
>> NH2-CGGGEGGGEGGGEGGGEGGGEGGGEG-CONH2
>>THEY ARE PREPARED BY FRAGMENT CONDENSATION OF TETRA PEPTIDES.
>>>PEPTIDE IS NOT VISIBLE AS A PEAK IN TFA(PH2) AND AMMONIUM ACETATE (PH 5.5)
> BUFFERS. IN AMMOUNIUM BICARBONATE BUFFER (PH 7) IN C18 9.4*250mm COLUMN IT
> HAS RETENTION TIME OF ABOUT 3.5MINUTES AND IT COELUTES WITH OTHER DELETION
> PEPTIDES OF MY SOLID PHASE SYNTHESIS.
>>>ANY SUGGESTIONS ON DIFFERENT BUFFER OR COLUMN CONDITIONS???
>>>THANKS,
>>SIVAKUMAR
>http://www.biowww.net/index.php/forum/forumlist/1/
