John Philo jphilo_nospam_ at mailway.com
Thu Jan 31 20:35:00 EST 2002

Antibodies are quite thermostable. The details will vary with the sequence
of the variable antigen-binding regions, the subtype, carbohydrate content,
and solvent conditions. The unfolding usually shows multiple transitions,
with different domains of the molecule unfolding at different temperatures.
Typically unfolding begins at ~60 C, the CH2 domain unfolds with a midpoint
of perhaps 75 C, and then the rest unfolds in a transition above 80 C.

A recent paper with details and references to other studies is Y. Mimura et
al. (2000) Molecular Immunology 37, 697-706.

'Hope this helps,

John Philo
Alliance Protein Laboratories

"Ahmed Samir" <ahmdsamir at link.net> wrote in message
news:a39net$16lea6$1 at ID-36824.news.dfncis.de...
> Hi there
> What is the tempreture at which the antibodies (G-Globulin) will be
> denatured??
> Thans so much
> Dr. Tamer Hassan

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