GFP purification short course

Daniel Gonzalez meton at rci.rutgers.edu
Thu Jan 31 16:55:03 EST 2002

I have been asked to announce the following,

                  The State University of New Jersey
                        Campus at New Brunswick

             Center for Research and Education in Bioluminescence
                          and Biotechnology
                   Programs in Biotechnology, Presents:

Protein Purification: Isolation, Analysis, and Characterization of GFP,
A Five and One-Half Day Hands-On Laboratory Course Using the remarkable
Green-Fluorescent Protein (GFP), A Novel Marker For Gene Expression,
as the source material

                            March 17-22, 2002

More than 800 scientists from around the world have strongly recommended this
intensive course as an opportunity to develop protein research and analytical
skills in a retreat setting.  Participants work hard, identify and solve
problems in the lab and enjoy camaraderie and good food and beer with

This five and one-half day laboratory course covers a wide variety of
conventional methods for protein isolation, purification, and
characterization.  The course format integrates hands-on laboratory exercises
with classroom lectures, demonstrations, study breaks, and short take-home

A special feature of the course is that all laboratory work will be performed
on the same starting sample (Aequorea GFP*), which will be purified from an
exceedingly crude form to near homogeneity as judged by high performance
liquid chromatography (HPLC), SDS gel electrophoresis, isoelectric focusing,
and capillary zone electrophoresis. This feature provides a continuity of
purpose, integrating dozens of preparative and analytical protein techniques
in a way that few competing courses can match.

A problem-solving approach will be used throughout the course.  Under the
guidance of experienced lab instructors, participants will work in groups
of three to plan their own protocols, analyze data, and interpret results.
A student-teacher ratio not greater than 8:1 will be maintained and the
faculty coordinators will be present throughout the course.

*Note: The use of GFP from a recombinant source (E. coli) is also being used
 as starting material due to its popularity within the scientific community.

For further details you can reach us,

by E-mail at: meton at rci.rutgers.edu
by phone at:  (732) 932-9071 extension 216
by FAX at:  (732) 932-3633

for a brochure and further information please visit the GFP purification
short course official Web site at:

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