Dear collegues!
I am working with purification of a recombinant His-tagged protein
using Ni-NTA column. I am wondering if there is any difference in
using imidazole vs. L-histidine for elution? Imidazole is cheaper
but, as I heard, it can influence activity of my protein, so
L-histidine is more recommended. Can it be so?
Thanx in advance
Nikolai
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Nikolai Scherbak
Orebro University
Fakultetsgatan 1
S-70182, Orebro, SWEDEN
tel.+46-19-301034
fax +46-19-303566
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