Tricks to eliminate non-specific binding of proteins to amylose r esin, any ?

"Khadka, Deepak NICHD khadkad at mail.nih.gov
Thu Jan 17 11:32:55 EST 2002

I am performing binding assay of some test proteins with MBP-fusion protein.
When I try to purify the mixture by using amylose resin, most of the test
proteins gets stuck to the resin. My buffer (both for binding and washing)
is 50 mM PIPES (pH 7.0), 100 mM NaCl and 1 mM EDTA. Playing with the salt
concentration and adding BSA did not help. Any suggestions ?



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