biotinylated 80bp-Fragment

Artem Evdokimov AEVDOKIMOZ at cinci.rr.com
Tue Jan 8 22:18:50 EST 2002

Well, 80-mers of DNA are expensive, no matter what. More, if you stick
biotin on them. Is your TF recombinant or natural ? How much TF do you
really need ?


"user" <q6252397 at fernuni-hagen.de> wrote in message
news:a1f4mh$11ic$1 at f1node01.rhrz.uni-bonn.de...
> Dear all.
> I would like to purify a transcription factor with DNA-Affinity
> chromatography.
> I bought a Streptavidin-Superflow Agarose resin. I'd like to couple
> biotinylated DNA-Fragments (80bp) on the column, to get active TF.
> The resin has a Biotin binding capacity of 350 nmol/ml resin.
> The problem is to get that much biotinylated DNA.
> Do you have any ideas for that? Any comments would be appreciated.
> TIA,
> Tina

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