Dear all.
I would like to purify a transcription factor with DNA-Affinity
chromatography.
I bought a Streptavidin-Superflow Agarose resin. I'd like to couple
biotinylated DNA-Fragments (80bp) on the column, to get active TF.
The resin has a Biotin binding capacity of 350 nmol/ml resin.
The problem is to get that much biotinylated DNA.
Do you have any ideas for that? Any comments would be appreciated.
TIA,
Tina
