Could you post some more details about the precise nature of the problem,
what protocol are you using etc.
A.G.E.
"koteshwar" <kkwar at yahoo.com> wrote in message
news:d1a3521b.0202270707.3c7a21cf at posting.google.com...
> I am Dr.koteshwar, working as a post doc. currently working on
> glutamine:D-fructose-6-phosphate amidotransferase of human over
> expressed in insect cells. I have a problem in purification of this
> enzyme since it looses its activity very rapidly.i would be grateful
> if anybody can give the suggestion to solve this problem.
> thak you
> kotesh
