Hi,
I'm doing exactly what you describe routinely. Just soak the stained gel
for 30 min in 1% SDS, pH 8.5, then do the electroblot. The nice thing
about that is that you can check the transfer by watching the coomassie
being blotted as well. Up to now, I haven't seen any interferences with
antibody detection.
Best regards,
Andreas
Qiang Qin wrote:
> Has anybody tried to eletroblot Coomassie stained gel directly to
> membrane then do Western? Will that work? Will the protein transfer
> well? And will the 1st antibody still react with the protein? Thanks.
>> Qin
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