Western blot on Coomassie stained SDS-PAGE gel?

Qiang Qin qqin at ccrc.uga.edu
Thu Feb 14 23:15:09 EST 2002

Has anybody tried to eletroblot Coomassie stained gel directly to
membrane then do Western? Will that work? Will the protein transfer
well? And will the 1st antibody still react with the protein? Thanks.



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