http://www.biochem.arizona.edu/montfort/research/abs/andersen2.htm
may come useful.
The idea is that you oxidize available thiol residues - if there's no thiols
there will be no crosslinking.
A.G.E.
"Madjid Hihi" <ahihi at po-box.mcgill.ca> wrote in message
news:3C61B4A1.28141369 at po-box.mcgill.ca...
> Diamide is used to oxidized many proteins but can we used this agent if
> there is a intramolecular disulfide bond in the monomeric state of the
> protein even if this protein is known to form dimer ? ...maybe the
> "artifactual" intramolecular -S-S- bridge obstruct diamide ???
>>nadoguy at hotmail.com>> ---
