In article <20021218065517.24337.qmail at ww02.hostica.com>, fxiong at asu.edu wrote:
>Hi, all,
>>I recently extracted overexpressed protein which was expressed probably in the
> form of inclusion body. I break the E. coli cells and spin down the pellet. It
> seemed kinds of difficulty to resuspend the pellet. Also, the suspensions are
> pretty jelly-like (Viscosity problem?) I'm wondering is this normal for
> inclusion body isolation?
Broken cells suspension with inclusion bodies looks milky
and pelleted inclusion bodies look like white/beige dense pellet.
Your jelly-like thing is most likely DNA, definitely not an inclusion bodies.
DK
