On 18 Dec 2002 fxiong at asu.edu wrote:
> I recently extracted overexpressed protein which was expressed probably in the form of inclusion body. I break the E. coli cells and spin down the pellet. It seemed kinds of difficulty to resuspend the pellet. Also, the suspensions are pretty jelly-like (Viscosity problem?) I'm wondering is this normal for inclusion body isolation?
>The jelly is likely DNA. If it bothers you, add some DNAse to break it up.
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legatek at mcmaster.ca Kyle Legate legatek at hotmail.com
Tower of Tongues:Thursday PM:10:30-11:30 EDT:http://cfmu.mcmaster.ca
moon musick:ritual:IDM:experimental(electronica):minimalism:glitch
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