groel copurification( masters needed)

[EK]

does "all buffers" include ones with low pH/salt/organic solvent
combinantions?
does "high salt" mean as well e.g. 1M MgCl2?

<cyril.boyault at ujf-grenoble.fr> wrote in message
news:20021210172019.2430.qmail at ww02.hostica.com...
> Dear all,
> I NEED help.
> I copurify groel with an histagged protein. The interaction between groel
and my protein is quite
> high because it  resit in all buffers tested ( atp containing buffers,
high glycerin ones, high salt,
> etc).
> So if anybody has even a tiny suggestion....
>
>
http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0