Im trying to measure an enzymatic activity in red cell lysates. it involves measuring citrulline as a product using a spectrphometer. however, because of the high haemoglobin content there is interference with the absorbance readings.
can anyone advise me how to get rid of the protein effect without affecting my enzyme activity?
http://biowww.net/mynews/tree.php?group_name=bionet_molbio_proteins&begin=0
