That was my original thought, too. However, I then noticed their catalogue
description: "Epitope mapping at the carboxy terminus of g-PAK p62 of human
origin (identical to corresponding rat sequence)". I checked more than one
POSTED human sequence against more than one POSTED rat sequence in the
database, and all show that 11 residues from the c-terminus human g-PAK
contains Methionine, whereas rat g-PAK contains Lysine. If I take Santa
Cruz's description as accurate then the epitope would not be from the
extreme c-terminus. Maybe it is wrong of me to assume they would not make
such a misprint, or perhaps they have different sequence data that I could
not find.
""Les Erickson"" <FLERICKSON at salisbury.edu> wrote in message
news:sd6cd829.015 at mail2.salisbury.edu...
> Santa Cruz's number system usually tells what the epitope was. For
> gamma-PAK C-19, I would bet that a peptide consisting of the very
> COOH-terminal 19 amino acids were used as the antigen.
>> Les
>> F. Les Erickson, PhD
> Assistant Professor
> Dept. of Biological Sciences
> 216 Henson Hall
> Salisbury University
> Salisbury, MD 21801
> (410) 677-5366
>http://henson1.salisbury.edu/~biology/faculty/Erickson.html>> >>> "-" <no.address at here.com> 08/28/02 12:18PM >>>
> I have been using an antibody raised against a sequence corresponding
> to (or
> near?) the c-terminus of human gamma-PAK (PAK I, PAK2). This antibody
> is
> catalogue #sc-1519 from Santa Cruz Biotechnology. I have contacted
> them
> inquiring about the residues this was raised against so I know where
> to
> focus on some interesting data I have obtained. However, they refuse
> to
> disclose the information. I have searched all journal articles I could
> find
> for any published information that could help, but turned up with
> nothing.
> (I even checked for any patented information). Does anyone know the
> residues this antibody recognized, or know of a place I can find
> reliable
> information?
>> Thanks in advance.
>>>
