I am using a DEAE 650M colum (Toyopearl) to fractionate ATP<ADP and AMP.
I already tried to precipitate the proteins by treating the sample with
20% Acetnitrile, but am not sure it is working that well.
I would appreciate if someone can give me more ideas as of how to
separate the proteins from bound nucleotides before loading the sample
onto the column.
Thanks in advance,
Guichy
