Detergents, nonionic for preference. BOG sometimes works miracles, but it is
just one out of thousands of other possibilities. Watch out for micelle
overconcentration.
A.G.E.
"Lisa Gabrielle Defazio" <ldefazio at stanford.edu> wrote in message
news:Pine.GSO.4.44.0208091028230.8153-100000 at saga21.Stanford.EDU...
>> Does anyone have suggestions on how to prevent my protein from sticking to
> the membrane when I dialyze it? I'm already adding loads of BSA, and my
> buffer contains 10% glycerol.
>> Thanks,
> Lisa
>